The SIET (Scanning Ion-selective Electrode Technique) can measure ion concentrations down to picomolar levels but must be measured slowly at approximately 1-2 seconds per point. This is mainly due to the mechanical disturbance of the gradient by the electrode movement, although the time constant of the LIX (Liquid Ion Exchanger) electrodes is also a factor. It takes a fraction of a second to reestablish the gradient. The electrode is stepped from one position to another in a defined sampling routine while also being scanned with the 3D micro-stepper motor manipulator (CMC-4). 1,2,3D or complex measurement paths are user programmable.
How does the SIET work? -see a movie clip
A complete SIET system contains:
  • RP-1 Research Platform 

  • IPA-2 Ion/Polarographic Amplifier with two ion heads 

  • 2HM-1 2 head mounting assembly with micromanipulator for simultaneous two electrode measurements

  • IEFS-1 Ion electrode filling station

  • Ion electrode kit - 2 teflon electrode holders, dri-ref. electrode

  • FC-1 Faraday cage

  • Sutter P-97  pipette puller

 



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